Published April 22, 2023 | Version http://biolifejournals.com/issueslist.php?cat_id=44
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Anti-Diabetic Activity of Selected Medicinal Plant Extracts Used By Tribals in the Adilabad District of Telangana State By in Vitro

Creators

  • 1. Department of Zoology, Osmania University, Hyderabad-500 007, Telangana State, India

Description

Abstract

Diabetes mellitus is a metabolic condition marked by high blood glucose levels as well as changes in carbohydrate, lipid, and protein metabolism. The goal of this study was to determine the inhibitory effect of selected plants (Artemisia vulgaris - root, and Angelica archangelica - leaves) on -glucosidase and -amylase to assess anti-diabetic effectiveness in vitro. By using the Soxhlet extraction method, each plant powder was repeatedly extracted with different organic solvents of increasing polarity. To investigate invitro anti-diabetic efficacy, the various solvent extracts were submitted to a -glucosidase and -amylase enzyme inhibition assay. Artemisia vulgaris stem bark crude extracts yielded 22.43 percent, 25.56 percent, 10.14 percent, 12.12 percent, and 1.10 percent in hexane, chloroform, ethyl acetate, acetone, and methanol, respectively. Angelica archangelica extracts were shown to have IC50 values of 57 g/ml, 43 g/ml, 63 g/ml, 64 g/ml, and 70 g/ml in n-hexane, chloroform, ethyl acetate, acetone, and methanol, respectively. In a dose-dependent way, all extracts reduced enzyme activity. Based on the IC50 values, Angelica archangelica chloroform extract was the most active of the two plant species, followed by Artemisia vulgaris. The chemicals responsible for A. archangelica promise in vivo anti-diabetic effect should be studied further.

Key words - Angelica archangelica, Artemisia vulgaris, alpha-amylase, alpha-glucosidase.

References

  1. Adisakwattana S, Lerdsuwankij O, Poputtachai U, Minipun A, Suparpprom C: Inhibitory activity of cinnamon bark species and their combination effect with acarbose against intestinal α -glucosidase and pancreatic α-amylase. Plant Foods Hum Nutr 2011, 66:143 – 148.
  2. Alberti KGMM, Zimmet PZ: Definition, diagnosis and classification of diabetes mellitus and its complications. Report of a WHO Consultation Geneva: WHO, 1999.
  3. Ali, H., Houghton, P.J., Soumyanath, A., 2006. -Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology 107, 449–455.
  4. Asano, N., Ikeda, K., Kasahara, M., Arai, Y., Kizu, H., 2004. Glycosidase-inhibiting pyrrolidines and pyrrolizidines with a long side chain in Scilla peruviana. Journal of Natural Products 67, 846–850.
  5. Conforti, F., Statti, G., Loizzo, M.R., Sacchetti, G., Poli, F., Menichini, F., 2005. In Vitro antioxidant effect and inhibition of α-amylase of two varieties of Amaranthus caudatus seeds. Biological and Pharmaceutical Bulletin 28, 1098–1102.
  6. E. Sucharitha and M. Estari. 2013. Evaluation of antidiabetic activity of medicinal plant extracts used by tribal communities in rural areas of Warangal district, Andhra Pradesh. Biology and Medicine, 5 (20-25).
  7. Gerrits PM, Tsalikian E: Diabetes and fructose metabolism. Am J Clin Nutr 1993, 58:796S – 799S.
  8. Ghosal S, Tripathi VK, Chauhan S. Active constituent ofEmblica officinalis: Part I. The chemistry and antioxidant effects of two new hydrolysable tannins, emblicanin A and B. Indian J Chem 1996;35:941-8
  9. ‐cyanogenic pyridone glucosides from Angelica archangelica (Euphorbiaceae). Phytochemistry 2009; 70 (2): 270‐277 
  10. Koh LW, Wong LL, Loo YY, Kasapis S, Huang D: Evaluation of different teas against starch digestibility by mammalian glycosidases. J Agric Food Chem 2010, 58:148 – 154.
  11. Kotowaroo, M.I., Mahomoodally, M.F., Gurib-Fakim, A., Subratty, A.H., 2006. Screening of traditional antidiabetic medicinal plants of mauritius for possible α-amylase inhibitory effects in vitro. Phytotherapy Research 20, 228–231.
  12. Lebovitz HE: Α-glucosidase inhibitors. Endocrinology and Metabolism Clinics of North America. 1997, 26:539-551.
  13. Lee, Y.A., Cho, E.J., Tanaka, T., Yokozawa, T., 2007. Inhibitory activities of proanthocyanidinis from persimmon against oxidative stress and digestive enzyme related to diabetes. Journal Nutritional Science Vitaminology 53, 287–292.
  14.  
  15. Pereira DF, Cazarolli LH, Lavado C, Mengatto V, Figueiredo MS, Guedes A, Pizzolatti MG, Silva FR: Effects of flavonoids on α -glucosidase activity: potential targets for glucose homeostasis. Nutrition 2011, 27:1161 – 1167.
  16. Porika Raju and Estari Mamidala (2015). Anti-diabetic activity of compound isolated from Physalis angulata fruit extracts in alloxan induced diabetic rats. The Ame J Sci & Med Res, 2015,1(1); Pages 1-6. doi:10.17812/ajsmr2015.11.1.
  17. Raj J. and Singh KP. Angelica archangelica. Central Council of Research in Homeopathic 2000; 22 (1&2)
  18. Rajendra Chary Vijayagiri and Estari Mamidala. Preliminary phytochemical and in vitro anti-diabetic activity of Ficus racemosa (L.) stems bark extract. Online International Interdisciplinary Research Journal, Voll-III, Nov 2013. 134-141
  19. Ramasubbu, N., Ragunath, C., Mishra, P.J., Thomas, L.M., Gyemant, G., Kandra, L., 2004. Human salivary α-amylase Trp58 situated at subsite-2 is critical for enzyme activity. European Journal Biochemistry 271, 2517–2529.
  20. Raptis SA, Dimitriadis GD: Oral hypoglycemic agents: insulin secretagogues, α-glucosidase inhibitors and insulin sensitizers. Exp Clin Endocrinol Diabetes 2001, 109(Suppl 2):S265 – 87.
  21. Sateesh Poojari, Raju Porika and Estari Mamidala. Phytochemical Analysis and in Vitro Antidiabetic Activities of Physalis angulata Fruit Extracts. National Journal of Integrated Research in Medicine (NJIRM) 2014; Vol. 5(2). 34-38.
  22. Sateesh Pujari and Estari Mamidala (2015). Anti-diabetic activity of Physagulin-F isolated from Physalis angulata fruits. The Ame J Sci & Med Res, 2015,1(1):53-60. doi:10.17812/ajsmr2015113.
  23. Schmelzer  GH.  Acalypha  indica  Linn.  Plant  Resources  Trop  Africa 2007.
  24. Scorpiglione N, Belfiglio M, Carinci F, Cavaliere D, De Curtis A, Franciosi M, Mari E, Sacco M, Tognoni G, Nicolucci A: The effectiveness, safety and epidemiology of the use of acarbose in the treatment of patients with type II diabetes mellitus. A model of medicine-based evidence. Eur J Clin Pharmacol 1999, 55:239 – 249.
  25. Sudhir R, Mohan V: Postprandial hyperglycemia in patients with type 2 diabetes mellitus. Treat Endocrinol 2002, 1:105 – 116.
  26. WHO Expert Committee on Diabetes Mellitus. Technical Report Series World Health Organization, Geneva, 1980.
  27. Zhang Y, Tanaka T, Iwamoto Y, Yang C, Kouno I. Phyllaemblic acid, a novel highly oxygenated norbisabolane from the roots of Phyllanthus emblica. Tetrahedron Lett 2000;41:1781-4

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